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KMID : 0859320100280030147
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Journal of the Korean Society for Therapeutic Radiology and Oncology
2010 Volume.28 No. 3 p.147 ~ p.154
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Combination Effect of Nimotuzumab with Radiation in Colorectal Cancer Cells
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Shin Hye-Kyung
Kim Mi-Sook
Jeong Jae-Hoon
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Abstract
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Purpose:To investigate the radiosensitizing effect of the selective epidermal growth factor receptor (EGFR) inhibitor nimotuzumab in human colorectal cancer cell lines.
Methods: Four human colorectal cancer cell lines, HCT-8, LoVo, WiDr, and HCT-116 were treated with nimotuzumab and/or radiation. The effects on cell proliferation, viability, and cell cycle progression were measured by MTT, clonogenic survival assay, flow cytometry, and Western blot.
Results:An immunoblot analysis revealed that EGFR phosphorylation was inhibited by nimotuzumab in colorectal
cancer cell lines. Under these experimental conditions, pre-treatment with nimotuzumab increased radiosensitivity of colorectal cancer cell lines, except for cell line HCT-116. However, cell proliferation or cell cycle progression was not affected by the addition of nimotuzumab, irrespective of irradiation.
Conclusions: Nimotuzumab enhanced the radiosensitivity of colorectal cancer cells in vitro by inhibiting EGFR-mediated cell survival signaling. This study provided a rationale for the clinical application of the selective EGFR inhibitor, nimotuzumab in combination with radiation in colorectal cancer cells.
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KEYWORD
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Colorectal cancer, EGFR, Nimotuzumab, Radiation, Combined therapy
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